Recently, the international regulations about the exhaust emissions of the motorcycle have been strengthened. The electrically controlled fuel injection type motorcycle has been emphasized to meet with this regulation. However, since the pulsation phenomenon happens in the intake port of the motorcycle because of the characteristic of high speed and the smaller layout than the passenger car, there are many difficulties to select the factor about control parameters needed to develop the ECU system. In this paper, the pulsation values measured from the engine test were compared with the calculated one by WAVE, and it was analyzed the pulsation characteristic according to the driving condition and estimated the mass flow rate. This research showed that the lowest point of the pressure gets lowin the low load and the pulsation of pressure were increased in the high load. Also, the simulation program was verified by showing good prediction of the pulsation and air mass flow rate.