Recently, a mesh-size insensitive structural stress definition that provides a stress state at weld toe with relatively larger mesh size compared to conventional approaches has been proposed. The structural stress definition is based on the elementary structural mechanics theory and provides an effective measure of a stress state in front of weld toe. In this study, as an experimental validation of structural stress method in obtaining the fatigue strength of weldments, a series of fatigue test has carried out for longi-web connections, which are representative of ship-like structures. Based on the result from this study, it is expected to develop a more precise fatigue strength evaluation technique and to reduce time and cost associated with the fatigue design of ship and offshore structures.