In this paper, we propose the location measurement algorithm of unknown tag based on RFID (Radio-Frequency IDentification) by using RSSI (Received Signal Strength Indication) and TDOA (Time Difference of Arrival) and extended Kalman filter in smart space. To do this, first, we recognize the location of unknown tag by using the RSSI and TDOA recognition methods. Second, we set the coordinate of the tag location measured by using trilateration and SX algorithm. But the tag location data measured by this method are included complex environmental error. So, we use the extended Kalman filter in order to revise error data of the tag location. Finally, we validate the applicability of the proposed method though the simulation in a complex environment.