We have studied the port-automation system which is requested by the steep increment of cost and complexity for processing the freight. This paper will introduce a new algorithm for navigating and controlling the autonomous Guided Vehicle (AGV). The camera has the optical distortion in nature and is sensitive to the external ray, the weather, and the shadow, but it is very cheap and flexible to make and construct the automation system for the port. So we tried to apply to the AGV for detecting and tracking the lane using the CCD camera. In order to make the error stable and exact, this paper proposes new concept and algorithm for obtaining the error is generated by the Virtual Photo-Sensor Array (VPSA). VPSAs are implemented by programming and very easy to use for the various autonomous systems. Because the load of the computation is light, the AGV utilizes the maximal performance of the CCD camera and enables the CPU to take multi-tasks. We experimented on the proposed algorithm using the mobile robot and confirmed the stable and exact performance for tracking the lane.