Purpose: The effect of two different levels of illumination and the effect of three letter chart types on subjective refraction findings were investigated. Methods: This study involved thirty Malay university students aged between 19 to 23 years old (7 males, 23 females), with their spherical refractive error ranged between plano to -7.75D astigmatism ranged from plano to -1.75D, anisometropia less than 1D and with no history of ocular injury and pathology. Monocular subjective refraction was measured under two levels of illumination (with and without room light) and with three different letter charts (Snellen letter chart, wall mounted letter chart and projected letter chart). Subjective refraction finding was calculated in spherical equivalent in unit diopter (D). Results: There was no significant effect in the subjective refraction findings with Snellen letter chart (t-test=0.15, p-value=0.88), projected letter chart (t-test=-0.19, p-value=0.85) as well as wall mounted letter chart (t-test=0.12, p-value=0.94). One Way ANOVA also revealed when the subjective refractive measures were compared under two different level of room illumination (with and without room light), no significant effect of letter chart types on subjective refraction readings with room light ($F_{2.185}$=0.11, p-value=0.89) and without room light ($F_{2.185}$=0.02, p-value=0.98). Conclusions: Subjective refraction findings were not affected whether the room light was on or off. They were also not affected by the types of letter chart used.