Purpose: The purpose of this study was to examine diurnal variation of accommodation on people whose age are between 35 and 50 years old with frequent alternation of accommodation at period 1 (8:00-10:00), period 2(13:00-15:00) and period 3 (18:00-20:00). Methods: All 4 subjects were separated to nonpresbyopia and incipient presbyopia. Tests were done for amplitude of accommodation, accommodative facility and relative amplitude of accommodation. Results: Both nonpresbyopia and incipient presbyopia had the highest amplitude of accommodation in oculus dexter, oculus sinister and oculus uterque at period 2. In addition, the incipient presbyopia showed the statistical significance of daily change. The nonpresbyopia had the most accommodative facility at period 3 and incipient presbyopia had the most accommodative facility at period 2. Their daily variations were statistically significant. Both nonpresbyopia and incipient presbyopia had the most negative relative accommodation of daily change at period I. Both of them had the most positive relative accommodation at period 2. Conclusions: The incipient presbyopia had the highest amplitude of accommodation, accommodative facility. and positive relative accommodation (except the negative relative accommodation) at period 2.