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Characterization of Three A-type Cyclin Genes in Tomato (Solanum lycopersicum) Treated with Auxins
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  • Characterization of Three A-type Cyclin Genes in Tomato (Solanum lycopersicum) Treated with Auxins
  • Characterization of Three A-type Cyclin Genes in Tomato (Solanum lycopersicum) Treated with Auxins
저자명
Guo. Jia,Kwon. Hye-Kyoung,Wang. Myeong-Hyeon
간행물명
Journal of the Korean Society for Applied Biological Chemistry
권/호정보
2010년|53권 3호|pp.266-274 (9 pages)
발행정보
한국응용생명화학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

We characterized three A-type cyclins from tomato: SlCycA1 (AJ243451), SlCycA2 (AJ243452), and SlCycA3 (AJ243453). All of them had the typical cyclin box and LVEVxEEY motif, and were homologous to other A-type cyclins. SlCycA1 and SlCycA2 were significantly expressed in meristematic tissues, but the transcript of SlCycA3 maintains relatively low levels in the various tissues we tested. To investigate the effect of various hormones on the expression level of A-type cyclins, two-week-old tomato seedlings were treated with $10;{mu}M$ indole-3-acetic acid (IAA), 3-Indolebutyric acid (IBA), or $alpha$-naphthalene acetic acid (NAA) for 0, 0.5, 1, 2, 5, or 10 d. Treatment with IAA or IBA significantly increased the cells in the G2 phase after treatment for 10 days, whereas the proportion of cells in the S phase was strongly increased by NAA treatment. RT-PCR analysis showed that the SlCycA1 and SlCycA2 transcripts accumulated at 5 days and 2 days after IAA treatment, respectively. Also, the SlCycA3 transcripts were strongly induced after 10 days of IBA or NAA treatment. These data provide new insights into the cell division accompanying hormone treatment in tomato.