To increase therapeutic efficiency and biological safety, it is important to precision control of acoustic output for therapeutic ultrasound equipment. In this paper, the electro-acoustic radiation conductance, one of electroacoustic characteristics of therapeutic ultrasound equipment, was measured by the radiation force balance method according to IEC 61161 standards and the acoustic output was estimated using the electro-acoustic radiation conductance. The estimation of acoustic output was conducted to continuous wave mode and pulse wave mode of duty cycle between 20% and 80%. The differences between prediction values and measurement results are within 5% of measurement uncertainty, which is a reasonably good agreement. The results show that acoustic output controlled by electro-acoustic radiation conductance was found to be an effective method.