The performance of emission factor has been validated by comparison with on-road test data. Emission factor, which is a function of vehicle speed, has been acquired based on chassis dynamometer test with NIER driving pattern. Portable Emission Measurement System, PEMS has measured on-road emission. Test vehicle was operated on defined test routes under different driving conditions, and made ten trips along its route. Emission factors properly simulate on-road test result, although there is some drawback to consider variety of driving condition on real world. Vehicle specific power and acceleration have been used to explain the distributed on-road result within same vehicle speed range. The trend in carbon dioxide and nitrogen oxide emission with respect to specific power and acceleration is clear. It has been found that specific power is a good explanatory variable for microscopic analysis for modal test result. Acceleration is good for microscopic as well as macroscopic analysis.