Purpose: The purposes of this study were to investigate the effectiveness of Pilates exercise on Fitness Factors related to motor performance including flexibility, agility, power, balance, and muscle endurance. Methods: Forty subjects were randomly allocated to one of two groups. The Pilates group did Pilates exercises 3 times a week for 8weeks, 60 minutes each time, and submitted to evaluation of protocols to assess sitting and reaching tests, a standing broad jump test, a side step test, a balance test using a Biodex Stability System, and muscle endurance using CSMI. Results: The Pilates group (n=20) participated in Pilates exercises three times in a week for eight weeks. The results show significant post-test differences in the Pilates group in the following areas: flexibility, agility, power, balance and muscle endurance. There was no significant increase in the control group. Post-exercise, there was no significant difference between the Pilates and control group. Conclusion: The Pilates method can offer significant improvement in personal flexibility, agility, power, balance, and muscle endurance. This study suggests that individuals can improve their Fitness Factors related to motor performance using Pilates exercises that do not require equipment or a high degree of skill. Further study is required to quantify the benefits of Pilates exercise.