Purpose: We investigated the changes in the stop-signal reaction time (SSRT) and the no-signal reaction time (NSRT) following motor sequential learning in the stop-signal task (SST). This study also determined which of the reduction0s of spatial processing time was better between blocked- and random-SST. Methods: Thirty right-handed healthy subjects without a history of neurological dysfunction were recruited. In all subjects, both the SSRT and the NSRT were measured for the SST. Tasks were classified into two categories based on the stop-signal patterns, the blocked-SST practice group and random-SST practice group. All subjects gave written informed consent. Results: In the blocked-SST group, both the SSRT and the NSRT was significantly decreased (p<0.05) but not significantly changed in the random-SST group. In the SSRT and the NSRT, the blocked-SST group was faster than the random-SST group (p<0.05). In the post-test SST after practice of each group, the SSRT was significantly decreased in the random-SST group (p<0.05), but the NSRT showed no significant changes in either group. Conclusion: These findings demonstrate that random-SST practice resulted in a decrease in internal processing times needed for a rapid stop to visual signals, indicating motor skill learning is acquired through improved response selection and inhibition.