This study was examined the effects of deep-sea water (DSW) supplementation and swimming exercise on bone metabolism in growing male rats. Thirty-two Sprague-Dawley male rats, 6 weeks of age, were randomly divided into high-fat diet sedentary (HS, n=8) or exercised group (HE, n=8) and DSW supplemented sedentary (DS, n=8) or exercised group (DE, n=8), given a high fat diet to all groups for 8 weeks. In the results, serum osteocalcin level of the DS was significantly lower than that of the HS (p<0.01). In addition, femoral bone mineral density (BMD), tibial bone mineral content (BMC), and breaking force of the DS were significantly higher than that of the HS (p<0.05). Furthermore, femoral BMD and tibial weight of DE were significantly higher than that of the HE (p<0.05). These results suggest that drinking deep-sea water and exercise has a crucial role for prevent osteoporosis and increase peak bone mass in growing male rats.