It takes long time in numerical simulation because structural design for tire requires the nonlinear material property. Neural networks has been widely studied to engineering design to reduce numerical computation time. The numbers of hidden layer, hidden layer neuron and training data have been considered as the structural design variables of neural networks. In application of neural networks to optimize design, there are a few studies about arrangement method of input layer neurons. To investigate the effect of input layer neuron arrangement on neural networks, the variables of tire contour design and tension in bead area were assigned to inputs and output for neural networks respectively. Design variables arrangement in input layer were determined by main effect analysis. The number of hidden layer, the number of hidden layer neuron and the number of training data and so on have been considered as the structural design variables of neural networks. In application to optimization design problem of neural networks, there are few studies about arrangement method of input layer neurons. To investigate the effect of arrangement of input neurons on neural network learning tire contour design parameters and tension in bead area were assigned to neural input and output respectively. Design variables arrangement in input layer was determined by main effect analysis.