To improve the sensing performance, cooperation among secondary users can be utilized to collect space diversity. In this paper, we focus on the optimization of cooperative spectrum sensing in which multiple cognitive users efficiently cooperate to achieve superior detection accuracy with minimum sensing error probability in multiple cross-over cognitive radio networks. The analysis focuses on two fusion strategies: soft information fusion and hard information fusion. Under soft information fusion, the optimal threshold of the energy detector is derived in both noncooperative single-user and cooperative multiuser sensing scenarios. Under hard information fusion, the optimal randomized rule and the optimal decision threshold are derived according to the rule of minimum sensing error (MSE). MSE rule shows better performance on improving the final false alarm and detection probability simultaneously. By simulations, our proposed strategy optimizes the sensing performance for each cognitive user which is randomly distributed in the multiple cross-over cognitive radio networks.