Purpose: The purpose of this study was to determine the factors associated with video display terminal (VDT) syndrome in students who use digital textbooks. Methods: A descriptive survey design was used, and 515 students were selected in 6 out of 20 designated as digital textbook research schools by the Ministry of Education, Science and Technology (MEST). Results: The subjective symptoms of VDT and their degree of severity in participants were similar to those in general students. Approximately 12.5% of the participants were classified as a potential risk group for VDT syndrome. In the potential risk group, the mean of the subjective symptoms of VDT was above normal ($2.53{pm}0.52$). Related factors were identified using logistic regression analysis and included being female (odds ratio [OR]= 2.57, p=.002), communication time with family (<30 min) (OR=2.70, p=.006),moderate satisfaction with school life (OR=2.57, p=.003),and dissatisfaction with school life (OR=14.92, p<.001). Conclusion: In order to prevent the occurrence of VDT syndrome in students, it is necessary to urgently establish a preventative program and monitor the use of digital textbooks.