To investigate the role of water temperatures and photoperidos as environmental cues regulating reproductive rhythm in banded catfish Pseudobagrus fulvidraco, rearing experiments were conducted using sveral rearing regimes conbined with changes in photoperiods and water temperatures during growing and spawning periods. GSI of $23^{circ}C$ was significantly higher than $18^{circ}C$ in female, but 9 light of 18 and $23^{circ}C$ were no difference in male. In case of estradiol, $18^{circ}C$ and 15 L was higher than other experintal precinct after 20 days. But, end of the experiment, all of experimental precinct were no difference. Testosteron of female was no difference in $18^{circ}C$-9 and 15 L after 20 days. Testosteron of male was no difference in $18^{circ}C$-9 L. In case of 11-KT, control level was $0.39{pm}0.03$ ng/mL and $18^{circ}C$-9 L was no significantly difference after 20 days. But, 15 L was higher than other experimental precinct.