The use of welding process has been increased for manufacture of machine, bridges, ships, gas facilities and so on together with development of welding technique. Accordingly, it has been needed to develop the welding methods considering higher productivity and safety design for manufacture of their welding structures. In this study, it was studied basically on characteristics of fatigue strength and fatigue life in full penetrated cruciform fillet weld zone in relation to material thickness, welding passes, loading direction and notch radius of toe zone. Most of fatigue failure occurred in toe zone of cruciform fillet weld joint. Fatigue strength and fatigue life are under the influence of stress concentration due to notch radius and flank angle of toe zone. The metal of toe zone annealed and diffused by multi-layer welding and acicular ferrite structure formed by the result improved fatigue strength and fatigue life.