Purpose: The purpose of this study is to identify the effects of the interpersonal relationship training program based on emotional intelligence on interpersonal relationship, emotional intelligence, and self-esteem of nursing students. Methods: The study adopted a quasi-experimental study with a nonequivalent control group pre-post test. Total participants were 89 nursing students of S and K Universities. They were divided into the control (39 students) and experimental group (50 students). Participants went through the emotional intelligence program 100min which consisted of 100 minutes per session, once a week for total of 8 session from March 3th to April 21th, 2011. The contents were composed with interpersonal relationship training program to promote positive formation of emotional intelligence, interpersonal relationship, and self-esteem. All data were analyzed by SPSS 18.0 program. Results: The experimental group showed a significant increase of emotional intelligence (t=-3.17, p=.002) and interpersonal relationship (t=-3.07, p=.003). Conclusion: The interpersonal relationship training program based on emotional intelligence was effective in improving emotional intelligence and interpersonal relationship.