Objectives : This study aims to investigate the relationship between the self-leadership and stress of students majoring in dental hygiene in clinical practice. This study will be helpful to cope with clinical practice effectively. Methods : Subjects were 221 dental hygiene students from 4 universities in Daegu and Gyeongbuk. Data were abalyzed by independent t-test and multiple regression analysis. Results : Stress-response was 60.36 points in high self-leadership group and 57.20 points in kow self-leadership group. It was statistically significant. Clinical practice-induced stress was made by self-expectation, rehearsal, and self-criticism. Stress was able to be overcome by self-reward and positive mind. Conclusions : It is necessary to take the curriculum into consideration which can improve the self-leadership before the clinical practice or at the early phase of clinical practice in order to ensure that the students majoring in dental hygiene are exposed to less stress arising from clinical practice and better cope with such stress.