Objectives : The purpose of this study is to investigate factors influencing on health promotion behaviors in public health course of college students. Methods : Subjects were 325 dental hygiene students and they completed self-reported questionnaires including health promotion behaviors, self efficacy, self esteem, and general characteristics from May 13 to 24, 2013. Students were recruited by sample convenience extraction. Data were analyzed using SPSS version 18.0 for descriptive statistics, t-test, one way ANOVA and multiple regression analysis. Results : Health promotion behaviors were closely related to concern for health care (p<0.001) and concern for health related goods (p<0.01). High self-efficacy and high self-esteem showed concern for health care (p<0.001). 2. Health promotion behaviors had significantly positive correlation with self efficacy, self esteem, and perceived health condition. 3. The most powerful predictor in health promotion behaviors was self efficacy. Self efficacy, self esteem, and major, concern for health-related goods, perceived health condition accounted for 40.8% of the total variance in health promotion behaviors. Conclusions : The result revealed that undergraduate students should take exercise and diet control for their health. Self-efficacy is the most important predictor of health promotion behaviors. Health promotion behavior programs should put emphasis on exercise and diet control and can be improved by behavioral change.