The ultimate goal of seismic design is to reduce the probable losses or damages occurred during an expected earthquake event. To achieve this goal, this study represents a procedure that can estimate annual loss probability of a structure damaged by strong ground motion. First of all, probabilistic seismic performance assessment should be performed using seismic fragility analyses that are presented by a cumulative distribution function of the probability in each exceedance structural damage state. A seismic hazard curve is then derived from an annual frequency of exccedance per each ground motion intensity. An annual loss probability function is combined with seismic fragility analysis results and seismic hazard curves. In this paper, annual loss probabilities are estimated by the structural fragility curve of steel moment-resisting frames(SMRFs) in San Francisco Bay, USA, and are compared with loss estimation results obtained from the HAZUS methodology. It is investigated from the comparison that seismic losses of the SMRFs calculated from the HAZUS method are conservatively estimated. The procedure presented in this study could be effectively used for future studies related with structural seismic performance assessment and annual loss probability estimation.