Optical nonlinear property is utilized to the wave generation, generating the beam at intended wavelength, and optical computing systems. The genetic material, which is the DNA with helical structure in nano-scale, is fascinating for optics communities due to artificially controllable sequence that determines the physical and chemical property. Nonlinearity of DNA was investigated by the four wave mixing experiment, which is with two incident beams located at 1550nm and 1650nm. The four output beams including incident beams are emitted from genetic material such as 1461nm and 1763nm by nonlinear characteristic. The 1461nm beam which is generated by four wave mixing phenomena was observed by optical spectrum analyzer.