Objectives: The purpose of this study was to provide substantial foundation to establish effective sex education plan for university students, and to inquire into sexual knowledge, consciousness and values of university students and into whether there are difference between health related major subjects and non-health related major subjects. Methods: This study was conducted on students in 227 health related major subjects and 226 non-health related major subjects in chungbuk C city. The Questionnaire, used in this study, was re-made on basis of reviews and previous studies, and total of 453 questionnaires were used. Results: Sexual knowledge scores were not significantly difference between health related major subjects and non-health related major subjects. but health related major subjects were more educated contraception, pregnancy artificial abortion, sexually transmitted disease than non-health related major subjects. Sexual knowledge was correlated with attitude in sex, times of sex education and usefulness of sex education. Conclusion: The university authorities make a curriculum for systematic sex educations and need to assertive support students and they can have right sexual consciousness and have mature and responsible attitude to sex through right education of knowledge in sex.