Objectives: The purpose of this study is to identify the relationship of personal hygiene education and hand-washing practices among adolescents. Then the impact of such factors on the hand-washing practices was analyzed. Methods: The data of the 2012 Youth Health Risk Behavior web-based Survey collected by Korean Center for Disease Control was analyzed using SPSS. Total 74,186 of middle and hish school students were included. Uni-variate analysis was done by complex sample crosstabs and multi-variate analysis was done by complex sample logistic regression. Results: The 26.8% of students experienced personal hygiene education. The students of boys, low school grade, coeducation, metropolitan, high school record and high economic status experienced more hygiene education. The hand-washing practices were high in the students with the experience of personal hygiene education. In the factors affecting the hand-washing practice, the experience of personal hygiene education was consistently significant. If students experienced the personal hygiene education, they showed 20~30% more rates of hand-washing practices. Conclusions: Hand-washing practice was high when experiencing personal hygiene education. The personal hygiene education was necessary to improve the rate of hand-washing practices.