This study has been carried out to understand the effect of Danchisoyosangagambang (DC) on the hyperglycemic mice induced with streptozotocin (STZ). Experimental groups were made diabetic mice by intraperitoneal injection of STZ (60 mg/kg of body weight) twice by 24 hr nterval and then 120 mg/kg STZ was injected again 3 days after the earlier treatment. Control group was administered mice with 0.9% saline (2 mL/kg), and experimental groups were administered DC extract (DCA group, 10 mg/kg/day; DCB group, 30 mg/kg/day) after hyperglycemic induction for 6 weeks. The body weight of experimental groups was lower than control. The blood glucose concentration increased continuously, reaching to 298.9 mg/dL after 6 weeks, however, experimental groups of the DCA and DCB groups significantly (p<0.01) decreased in the 4, 5, and 6 weeks groups. Blood glucose tolerance test was not significant between control and experimental groups. We examined the blood transaminase activities to know the effect of herbal medicine on liver function. The GOT activities were lower in group DCB than in control. The GPT activities were lower in group DCA and DCB than in control. The results of immunohistochemical study, a few of insulin positive cells observed in the control and experimental group. These results suggest that administration of DC extract to the hyperglycemic mice decreased the blood glucose level.