This study aims to examine self-esteem in health and non-health college students and their attitude toward the disabled. This study investigates the effects of their self-esteem on their attitude toward the disabled. The data for this study were collected from March 2 to 12, 2011. For data collection, we used a structured questionnaire to students at one emergency medical technology department and 3 engineering departments of at three colleges with their consent after explaining the purpose of this research. A total 703 questionnaires were collected and used for analysis. Rosenberg Self-Esteem Scale (RSES) developed by Rosenberg (1965) and translated into Korean by Jeon Byeong Jae (1974) was used to measure self-esteem and a questionnaire with six areas of 37 items revised and complemented by Lee Jong Nam (1994) was based on Disablity Factor Scale (DFS) with seven sub-factors of 43 items developed by Siller (1967) was employed to measure their attitude toward the disabled. Data collected were analyzed with cross analysis, technical statistics, t-test, ANOVA, Pearson’s correlation coefficient and regression analysis using SPSS PC/12.0. The results of statistical analysis were summarized below. 1) As a result of comparing self-esteem between the two groups, it was found that health college students were higher (3.83) than non-health college students (3.68) (p=0.013). 2) Attitudes toward the disabled was higher in health college students (2.78) than in non-health college students (2.55) (p=0.032). The mean score in six sub-factors was higher in health college students and there were significant differences in inferred emotional disorders (p=0.048) and contact tension (p=0.010). 3) Differences in self-esteem according to general characteristics and disability related characteristics showed that there were significant differences in grade (p=0.016), gender (p=0.000), age (p=0.000), and presence or absence of the disabled around them (p=0.003) in health college students and in gender (p=0.000), age (p=0.001), and their interests in disability (p=0.000) in non-health college students. 4) Attitudes toward the disabled according to general characteristics and disability related characteristics showed that there were significant differences in age (p=0.011), presence or absence of the disabled around them (p=0.045), their interests in disability issue (p=0.016), and their image of the disabled (p=0.014) in health college students and in their interests in disability issue (p=0.008) and their image of the disabled (p=0.041) in non-health college students. 5) There were positive correlations in self-esteem and attitudes towards the disabled between health college students (r=0.438) and non-health college students (r=0.348). 6) Influence of self-esteem on attitude to the disabled was 29.8% in health college students and 25.7% in non-health college students. To sum up the above results, it was found that there were significant positive correlations in self-esteem and attitudes toward the disabled between health and non-health college students. The higher self-esteem, the more positive the attitude towards the disabled. Therefore, it is concluded that more systematic and effective educational programs should be developed in consideration of the influence of self-esteem in health and non-health college students on their positive attitude to the disabled.