This study was conducted to explore the prevalence of sexual dysfunction and to determine the related factors influencing sexual function in peri-menopausal women. A total of 236married women aged 20-59 years participated in this study. Data were collected through self-report questionnaires which were constructed to include general characteristics items, depression, and female sexual function. The mean total sexual function score was 19.49 while the proportion of women with female sexual dysfunction was 61.4%. Significant predictors influencing sexual function in peri-menopausal women were hypertension, sexual partner's age, frequencies of pregnancy and delivery, and depression, and these predictors account for 37.9% of the variance in sexual function. We propose the development of sex-related counseling and education program that can improve quality of life and sexual function based on the related factors that may affect sexual function in peri-menopausal women.