Objective: As a method to develop regular daily routines and affection at nursery schools, we prepared “facial expression stickers" and “health handbooks" and investigated their efficacy by conducting an intervention study. In the present study, we ascertained whether or not facial expression stickers would be useful in developing affection between nurserγ school children and teachers. Methed: Facial expression stickers were developed so that nursery school children could keep regular daily routines the regular use of stickers and could examine the state of their own mind and body. In order to observe the changes in affection between nusery school children and teachers, questionnaires were prepared. The intervention study was conducted from September to the end of November in 2008 by establishing the following two experimental groups: the long-term intervention group (for 2.5 months, n=131) and short- terrn intervention group (for 0.5 months, n=134). The results of the teachers’ affection assessment questionnaires were ana1yzed. Results: Before intervention, the affection assessment score for 3- and 5-year-olds was significantly higher in the short-terrn intervention group than in the long-term intervention group. However after intervention, the affection assessrnent score for 3- year-olds was significantly higher in the long-term intervention group than in the short-term intervention group. For the changes before and after intervention, the affection assessment score significantly increased for all age groups after the long- terrn intervention. However, after the short-term intervention, the affection assessment score showed no change for 4- and 5-year-01ds and significantly decreased for the 3-year-olds. Conclusion: The results showed that the development of affection was greater using facial expression stickers for 2.5 months than using facial expression stickers for 0.5 months at the nursery schoo1, and long-term use of facial expression stickers may be useful to develop affection between teachers and nursery school children