This study was conducted to analyse the muscular activation and the MIDI-keyboard pressing strength (velocity) in order to investigate differences in hand function of visually impaired elderly women depending on the pressing patterns (sequential/separating). Participants were 10 visually impaired women aged more than 65 years who used services of the welfare center in S city and voluntarily agreed to participate in the study. Each participant performed both keyboard pressing patterns with surface electrodes on their finger flexor and extensor muscles. During each of two playing conditions, activations of finger flexor and extensor muscles were measured from the EMG equipment (LXM 5380) and keyboard pressing strength was collected via the velocity data from the Cubase program. Results of this study showed that the muscular activation was significantly higher in both finger flexor and extensor muscles when performing the sequential pressing than separating pressing. On the contrary, MIDI-keyboard pressing strength marked the higher scores in the separating pattern than in the sequential one. This implies that the muscular activation could be influenced more by the pressing pattern than by the sound intensity to be produced. These findings indicate that the pattern of keyboard playing should be considered for hand function improvement of visually impaired elderly women and the sequential pressing could be more efficient for muscular activation.