Self-injury is one of the leading causes of suicide, it is known to cause several negative consequences in individuals. Self-injurious behavior even occurs in high-functioning individuals who do not have clinical problems. In this study, 994 middle and high school students having experiences in non-suicidal self-injury were surveyed to find out the relationship among shame proneness, guilt proneness, frequency and severity of nonsuicidal self-injury. The Functional Assessment of Self-Mutilization and the Test of Self-Concern Affect-adolescent were used for the research tools. The result shows shame proneness and guilt proneness predicted non-suicidal self-injury, moderate/severe level of non-suicidal self-injury showed a negative correlation with guilt proneness, and nonsuicidal self-injury of mild level showed a significant positive correlation with shame proneness. In addition, there was no significant difference in guilt proneness and shame proneness between the moderate/severity level and mild level non-suicidal self-injury group, but the moderate/severe non-suicidal self-injury group showed a tendency to have a lower guilt proneness score compared to the other group. In other words, this study confirmed that should also considered account guilt proneness when intervening in the non-suicidal self-injury group at the moderate/severe level. At the end, the significance and limitations of this study were discussed, and suggestions for future research were included.