The purpose of this study is to investigate the relationship between Hippocrates substrate type(DISC) of nursing students, Clinical performance ability and Clinical performance ability. This study was implemented under explanation of purpose and agreement of a total of 300 students attending in nursing students. The subjects are nursing students in 3rd and 4th grade. The analysis was done using SPSS 23.0win program. As a result, the Hippocrates substrate type(DISC) of nursing students had the highest mucus(stable). the Clinical performance ability was 3.68±.56, and the Stress in clinical practice was 3.38±.64. Looking at the relationship between DISC, Clinical performance ability, and Stress in clinical practice, Among the Hippocrates substrate types, bile duct (leading type) scored 3.86±.46, indicating that the Clinical performance ability was the highest. In relation to clinical stress, the clinical stress was 3.47±.55 in depressive (new medium), but it was not statistically significant. As a result of this study, there was a difference in Clinical performance ability according to Hippocrates substrate type(DISC),it is considered to be the basic data for preparing various strategies for improving Clinical performance ability according to Hippocrates substrate type(DISC).