The purpose of this study was to explore the effects of flipped learning strategies on pre-service teachers’ collective self-esteem and attitude toward cooperative learning in design thinking learning environments. For this purpose, this study conducted an experiment for 69 pre-service teachers who took the classes of Educational Technology-related course in D university of Busan Metropolitan City for 15 weeks on spring semester of 2019. In order to examine whether there were any significant differences between their collective self-esteem and attitude toward cooperative learning the type of flipped learning strategies in the design thinking learning environments, the pre-tests and post-tests of the pre-service teachers’ collective self-esteem and attitude toward cooperative learning were conducted for the subjects. Later, multivariate covariance analysis(MANCOVA) was used to examine the differences among the groups. The main results of this study are summarized as follows. First, there were no significant differences in the means of the collective self-esteem according to the flipped learning strategies. Second, in the case of the pre-service teachers’ attitude toward cooperative learning, there were significant differences in the means according to the flipped learning strategies. In addition, it was found that in the three of the four sub-factors for attitude toward cooperative learning, there were significant differences among the groups. Based on the results of this study, teacher training institutions should specify the flipped learning strategies that can positively affect the pre-service teachers’ collective self-esteem and attitude toward cooperative learning using design thinking learning environments, this may be applied to develop instructional design and used to improve the professional skills of pre-service teachers through field experiences.