This study was done to investigate the effect of the first experience of the clinical experience for psychiatric nursing on urinary Na+, Cl-, K+, Ca++ of the nursing students. We analyzed the urine of 36 students on curriculum who were students of D college in K city. The data were analyzed with SAS Statistical analysis was performed by using paired t-test, GLM. The second day group increased 18.56 at 8AM, 31.90 at 4PM in a case of Na+(p=0.004). The second day group increased 27.61 at 8AM, 43.53 at 4PM in a case of Cl-(p=0.009). The first day group increased 2.62 at 8AM, 7.09 at 4PM in a case of K+(p=0.018). The second day group increased 3.69 at 8AM, 5.19 at 4PM in a case of K+(p=0.013). The second day group increased 20.65 at 8AM, 14.07 at 4PM in a case of Ca++(p=0.033). There was a significant difference in Na+ according to group at 8AM(F=4.17, p=0.024) and 4PM(F=3.58, p=0.040). There was a significant difference in Cl- according to group at 8AM(F=4.38, p=0.020) and 4PM(F=6.29, p=0.003). There was a significant difference in K+ according to group at 8AM(F=5.03, p=0.012). In conclusion, Na+, Cl-, K+, Ca++ may be used as a indicator of the amount of stress to improve the educational environment for the students.