운동과 칼로리 제한에 따른 비만유도 쥐의 Sirtuin과 심부온도 변화에 관한 연구

서지반출

국문초록

본 연구는 12주간의 유산소 운동과 칼로리 제한이 비만유도 쥐의 시르투인(SIRT1) 발현과 심부온도 변화에 어떠한 영향을 미치 는 지를 알아보는 것이다. 본 실험대상 동물은 C57BL/6계 수컷 쥐 40마리를 고지방식이(탄수화물 22%, 단백질 18%, 지방 60%)에 의해 비만을 유도하여, 통제군(Con), 칼로리 제한군(DG), 운동군(EG), 복합처치군(DEG)으로 나누어 실험하였다. 칼로리 제한 방법 은 실험 실시 1주 전 모든 집단의 하루 총 섭취식이평균량을 측정하여 그 평균량의 60%를 적용하였고, 운동 방법은 최대심박수 40~50%의 강도(9~10m/min)로 주 5회, 매 회당 30분, 12주 간 수행하였다. 심부온도의 측정은 실험 실시 전?후 모든 집단의 직장을 통하여 1회씩 측정하였고, 시르투인(SIRT1) mRNA는 역전사 반응과 Real-time PCR 방법을 사용하여 발현량을 측정하였다. SIRT1 mRNA 발현에 대한 차이를 분석한 결과는 집단 간 통계적으로 유의한 차이가 있는 것으로 나타났다(p<0.01). 사후검증을 실시한 결과 통제군(Con)에 비해 운동군(EG)과 복합처치군(DEG)에서 발현량이 유의하게 높게 나타났다(p<0.01). 심부온도 변화에 대한 분 석결과는 심부온도의 집단 내 전?후 변화를 분석한 결과 복합처치군(DEG)에서 유의한 차이를 나타내며 심부온도가 감소되었다 (p<0.05). 이 연구를 통하여 노화와 비만억제 및 수명연장에 긍정적 영향을 미치는 시르투인(SIRT1)의 발현과 심부온도 감소를 위 한 가장 효과적인 방법은 유산소 운동과 칼로리 제한의 복합처치라고 사료된다.

영문초록

This study was to investigate an influence of aerobic exercise and calorie restriction on a sirtuin gene (SIRT1) and core body temperature. The mice were separated into 4 groups: control(Con), calorie restriction(DG), exercise(EG), calorie restriction & exercise(DEG). During the period of adaptation prior to the experiment, dietary-induced obesity consumption (high-fat diet: 60%)was set up as the standard. Aerobic exercise was moderately assigned by 9~10m/min, 5 sessions a week for 12 weeks. Insertion of a thermometer was used to measure core body temperature through the anus of mice before/after the experiment. The results showed that moderate aerobic exercise had a positive effect on sirtuin gene and it was confirmed that calorie restriction is useful to decrease core body temperature. Thre result of the expression of SIRT1 mRNA was significant in between groups(p<0.01). Particularly the expression of EG and DEG groups were higher than Con(p<0.01). The change of core body temperature was significantly decreased in DEG group(p<0.05). The overall conclusion is that both moderate aerobic exercise and calorie restriction might be helpful to reduce obesity and extend lifespan in accordance with higher SIRT1 and lower core body temperature.

키워드

exercise caloire restriction sirtuin core body temperature

참고문헌 (48건)

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