한국산 호박씨중에서 지방(脂肪)을 추출하여 지방산(脂肪酸)의 종류를 조사하였고 또한 물에 가용(可溶)인 단백질(蛋白質) 및 불용성(不溶性) 단백질(蛋白質)을 분리(分離)하여 Disc 전기영동으로 단백질의 Pattern을 확인하고 아미노산 조성(組成)을 관찰하였다. 1. 호박씨의 일반성분중 지방은 46.5% 단백질 25.5%를 함유하고 있다. 2. 호박씨기름의 지방산(脂肪酸) 조성(組成)은 linoleic acid 51.74%, oleic acid 22.2%, palmitic acid 18.1%, stearic acid 7.84%로 이들 4가지를 주 지방산으로 하고 있다. 3. 호박씨 단백질(蛋白質)의 아미노산(酸) 조성(組成)은 tyrosine, glutamic acid, aspartic acid, leucine, glycine등을 다량(多量) 함유하고 있으나 함황(含黃) 아미노산(酸)은 함유(含有)하고 있지 않다. 4. 단백질의 Disc전기영동 결과 가용성(可溶性) 단백질(蛋白質)에서 13개의 band와 불용성(不溶性) 단백질에서 4개의 band가 분리 되었다.
Dehulled pumpkin seeds produced in Korea were defatted and their composition of fatty acid was analyzed by gas chromatography. Soluble protein and insoluble protein were isolated from the dehulled and defatted pumpkin seeds. The protein component was identified by disc-electrophoresis and the amino acid composition of the protein was analyzed. (1) The pumpkin seed contained 46.5% of fat and 25.5% of protein respectively. (2) The fatty acid of the pumpkin seed was compassed chiefly of linoleic acid (51.74%), oleic acid (22.2%), palmitic acid (18.1%) and stearic acid (7.84%) (3) The amino acid composition of the soluble and insoluble seed protein contained most of all the amino acids except for S-contained amino acids. (4) It has been identified by disc-electrophoresis that the soluble protein had 13 bands and the insoluble protein had 4 bands.
