This study analyzed the policy effect of ‘school start at 9’ in Gyeonggi province since September 2014 on sleep hours and tiredness among adolescents, employing the difference in differences (DID) analysis. 2014 Time-use Survey in Korea administered by Statistics Korea was used to conduct the analysis. The findings showed the immediate increase in sleep hours right after introducing the delayed school start time. However, the policy effect became non-significant in three months since the policy introduction because adolescents adjusted their life-cycle to school start time at 9. Study hours had a negative effect on sleep hours, and thus, we speculated that the effect of the delayed school start time on sleep hours was likely to be mediated by the adjustment of study hours. Tiredness adolescents felt significantly decreased after the delayed school start time, and the policy effect on tiredness was still effective after three months since policy introduction. The results may be because delayed school start time had led their bedtime and wake-up time more appropriate to typical biorhythm among adolescents.