This study investigated the mediating effect of perceived calling in a relationship between the spiritual maturity and career maturity of Christian college students. 384 Christian college students participated in this study, and the results showed that more mature students tended to show more mature career preparation. Furthermore, this study examined the mediating effect of perceived calling in a relationship between spiritual maturity and career maturity. The results indicated that perceived calling fully mediated the relationship. In other words, those who are spiritually mature tend to have a greater sense of perceived calling, which leads to high career maturity. The results implied that Christian college students will gain higher perceived callings resulting from greater spiritual maturity, and this will lead them to develop career maturity. Therefore, it is important to create an educational program that helps Christian college students finding their callings and follow their callings so that they might have a more mature career preparation attitude once they choose a career path.