The purpose of this study was to compare the sleep and fatigue between groups with two shift and three shift nurses. A total of 14 nurses working in Neonatal Intensive Care Unit(NIC) were included with the seven for two shifts and the other seven for three shifts. Data were collected by Korean version of sleep tool(Oh et al., 1998) and fatigue symptom tool (Kim et al., 2001) and analyzed using SPSS PC 12.0. The results of the study were as follows: 1) There was statistically a significant difference in the number of total sleep hour and no difference in fatigue between groups. 2) There were no significant differences found in both sleep and fatigue by demographic characteristics between groups. 3) There were no correlations between sleep and fatigue in both groups. Through the further future studies, the findings of this study were expected to provide an opportunities for exploring the information on 2-shift nurses as well as 3-shift nurses and to offer the fundamental data on an arrangement of work force according to the types of shift work.